Ambrosio has made several television appearances, notably a cameo as herself on HBO's Entourage along with Izabel Goulart; The Late Late Show with Craig Kilborn; Late Night with Conan O’Brien; as guest judge on Project Runway season 2: Team Lingerie and The Tyra Banks Show where she was a guest twice. She appeared alongside TV personality, Regis Philbin in a worldwide commercial for Hummer H2. In 2006, she had a cameo on the box-office hit movie, Casino Royale appearing briefly as Tennis Girl #1.
Ambrosio guest-starred on How I Met Your Mother (episode: "The Yips") on November 26, 2007 with her fellow Victoria's Secret Angels Adriana Lima, Selita Ebanks, Marisa Miller, Miranda Kerr, and Heidi Klum.
Ambrosio guest-starred on How I Met Your Mother (episode: "The Yips") on November 26, 2007 with her fellow Victoria's Secret Angels Adriana Lima, Selita Ebanks, Marisa Miller, Miranda Kerr, and Heidi Klum.